El cultivo de protoplastos en cítricos y su potencial para el mejoramiento genético. Article (PDF Available) · July with 1, Reads. Export this citation. Results 80 – 90 Download Citation on ResearchGate | On Jan 1, , R.R. Rodríguez and others published Aislamiento y cultivo de protoplastos en maracuyá. Universidad de León – Grado en Biotecnología – Tercero Biotecnología – Biotecnología vegetal – Tema 9. Cultivo de protoplastos e hibridación somática.

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Microcalli were transferred onto MS medium to induce shoot regeneration under indirect light conditions. Acknowledgments The authors would like to thank the International Potato Center for financial support and to Martha Williams de Castro who revised the English version of this article.

This study pgotoplastos that somatic hybrid potato plants can be obtained by the fusion method presented. When colonies developed into microcalli, the alginate beads were depolimerized. Then the suspension was centrifugated at rpm during ten minutes.

Tema 9. Cultivo de protoplastos e hibridación somática

Chromosome observations indicated that all hybrids were aneuploids. Root tips were stained by lacto-propionic orcein. Avoid resits and achieve higher grades with the best study guides, textbook notes, and class notes written by your fellow students. After seven days, the culture medium was diluted 1: Transfer of resistance to potato leaf roll virus from Solanum brevidens into Solanum tuberosum by somatic fusion.

These barriers in classical breeding can be overcome using biotechnological methods such as somatic hybridization by protoplast fusion Carputo et al. A rapid calli proliferation was observed and most of them showed purple-colored cells Fig. A revised medium for rapid growth bioassays with tobacco tissue culture. Potato, Solanum tuberosum L. These plants were multiplied in glass culture tubes for further analysis. Plant Cell Rep 3: Earn hundreds of dollars each month by selling your written material to your fellow students.


The other parent, the wild species S. Somatic potato hybrids have been produced by fusion of diploid wild species protopladtos tetraploid S. Somaclonal variation in potato. Aggregations of more than two protoplasts were also observed. Characterization of somatic hybrids of potato by use of RAPD markers and isozyme analysis. These samples were obtained from rooted plants maintained in a greenhouse. This value is different from that obtained by Mattheij et al.

Plant Cell Tissue Organ Cult. Protoplasts were precipitated by centrifugation at rpm during five minutes. Results and discussion Protoplasts fusion and plant regeneration Protoplasts fusion from S.

Shoot regeneration started twelve weeks after calli were transferred to MS medium. Tissue culture response in various wild and cultivated Solanum germplasm accessions for exploitation in potato breeding.

Isolamento e cultivo de protoplastos de porta-enxertos de citros

In this paper we describe the fusion of leaf protoplasts of S. Plant Cell Rep Non-transgenic applications of plant tissue protolastos in potato.

This technique has created novel cellular genome configurations by combining sexually incongruent species. The morphology of fusion-derived regenerants was intermediate between the donor parents. Prior to fusion, protoplasts from S. This can be done via morphological, biochemical, cytological and molecular markers Pinto et al. To select hybrids, regeneration of in vitro plants was subjected to RAPD analysis.

Nineteen somatic hybrid plants were obtained and confirmed by RAPD analysis. Production of somatic hybrids between frost – tolerant Solanum commersonii and Solanum tuberosum: RAPD and chromosomal analysis of the hybrids are also presented. After protoplasts fusion and during plant regeneration it frequently happens that genetic changes such as variations in chromosome number occur.


Interspecific hybridization between the cultivated potato Solanum tuberosum subspecies tuberosum L. These results showed that all hybrids were aneuploids Fig.


rpotoplastos Using chemical or electrical procedures Jones, protoplasts from different donor plants can be fused together and somatic hybrids regenerated from the fusion products.

Many wild Solanum species are regarded as important sources for disease resistance and tolerance to many abiotic stresses Hawkes, but their use in potato breeding is limited due to poor crossability and sterility of interspecific hybrids.

A rapid calli proliferation was observed after colonies developed. The enzyme treatment was carried out overnight with gentle agitation. Segundo curso Primer curso.

Thanks to the study guides written by fellow students specifically for your courses, you will never miss a trick when it comes to your exams. In conclusion, somatic fusions between the cultivated potato Solanum tuberosum and the wild species S. For these reasons and considering the obtained results, it can be suggested that there is a high probability of fusion-derived regenerants which are aneuploids with an increase or loss of some chromosomes when one or both fusion partners have a high ploidy level.

Successful application of the fusion technique demands a protocol for plant regeneration from protoplasts. Isolation of plant DNA from fresh tissue. Interspecific somatic hybridization between Solanum tuberosum L.