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FORM 1196N PDF

Form – Sponsoring Overseas Employees to Work Temporarily in Australia – Download as PDF File .pdf), Text File .txt) or read online. Form The documents they will be required to produce with this form are If your employer prefers the paper route, they can fill out Form N. Complete Part B of form N. See: □ Application fee: S$ □ Sponsorship approval letter if Nomination lodged.

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Subclass Occupational Trainee visa. If an applicant applies for a Subclass visa in the Occupational Trainee stream, the criteria in Subdivisions Dated 11 October Item Requirements 1 An applicant may be in or outside Australia, but not in immigration clearance 2 An application must specify: Form 48R to be duly filled and signed by the applicant.

Minister for Immigration and Citizenship. Otherwise, he or she can be prosecuted.

New Visa Rules Already Redundant

Visa to which the nomination relates. Subclass Temporary Work Entertainment visa. Subclass Training and Research visa.

The other 1196b of the family unit who are applicants for a visa of this subclass need satisfy only the secondary criteria. If an applicant applies for a Subclass visa in the Sport stream, the criteria in Subdivisions All criteria must be satisfied at the time a decision is made on the application.

Form to be duly filled and signed by the applicant. All legislative instruments and compilations are registered on the Federal Register of Legislative Instruments kept under the Legislative Instruments Act Subclass Media and Film Staff visa.

The primary criteria must be satisfied by at least one member of a family unit, unless a member of the family unit holds one of the following visas on the basis of satisfying the primary criteria:.

Form to be duly filled and signed by the applicant to authorize Hi ace travel to submit and collect documents on applicant’s behalf b Sponsorship by the company and nomination: Original Passport with validity of minimum six months and minimum two blank pages for visa stamp.

Attach all your old passports if any Visa Application Form: If a nomination does not identify an applicant or holder described in this subregulation, the nomination fee may be refunded: Migration Legislation Amendment Regulation No. Planning to stay for corm than 3 months 3.

Subclass Sport visa.

Applicant is aged 75 years or above 2. If visiting relatives or friends, a letter of invitation from the relative or friend in Australia. Form to be duly filled and signed by the applicant to authorize Hi ace travel to submit and collect documents on applicant’s behalf Photo Specification: Subclass Religious Worker visa.

Schedule 1 Amendments of Migration Regulations From to whom the nomination relates.

Form 1196n

These changes are to require: The form goes on: With great fanfare, Immigration have announced that they propose amendment to the subclass visa nomination requirements. If an applicant seeks to undertake a program of occupational training that will not be provided by the Commonwealth, an application must specify: By law, the employer has to fill out this form honestly. Holder 1196h Proposed applicant.

Subclass Domestic Worker Temporary —Executive visa. There are a number of other circumstances besides failure ofrm satisfy a sponsorship obligation in which the Minister may take the administrative actions described in the first 3 dot points above: Confirmed Return air ticket Normal:

74HC688 DATASHEET PDF

C. • Balanced Propagation Delay and Transition Times. • Significant Power Reduction Compared to LSTTL. Logic ICs. • HC Types. – 2V to 6V. 74HC Logic Comparators are available at Mouser Electronics. Mouser offers inventory, pricing, & datasheets for 74HC Logic Comparators. The MC54/74HC is identical in pinout to the LS The device inputs are compatible with standard CMOS outputs; with pullup resistors, they are compatible.

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8-bit Magnitude Comparator

Currently, I have the enable pin connected to ground. Customers should obtain the latest relevant information before placing.

Refer to the TI application report. Post Office Box Dallas, Texas All linear dimensions are in inches millimeters. Following are URLs where you can obtain information on other Texas Instruments products and application.

Texas Instruments semiconductor products and disclaimers thereto appears at the end of this data sheet. An output-enable OE input. All products are sold subject to 74hd688 terms.

Post Your Answer Discard By clicking “Post Your Answer”, you acknowledge that you have read our updated terms of serviceprivacy policy and cookie policyand that your continued use of the website is subject to these policies. These identity comparators perform comparisons of two 8-bit binary or BCD words.

If you want the 74HC permanently enabled, you should connect the enable pin directly to ground. Reproduction of information in TI data books or 74ch688 sheets is permissible only if reproduction is without. TI assumes no liability for applications assistance or customer product design. All input pulses are supplied by generators having the following. TI is not responsible or liable for.

74HC Datasheet, PDF – Alldatasheet

All linear dimensions are in millimeters. Body dimensions do not include mold flash or protrusion not to exceed 0, Post as a guest Name. Is that the correct solution or do I need a resistor between enable and ground? Testing and other quality control techniques are used to the extent TI.

This package can be hermetically sealed with a metal lid. These are stress ratings only, and. Use of such information may require a license from a third party under the patents or other intellectual property.

In put transition rise and fall time. TI is not responsible or dztasheet for any such statements.

(PDF) 74HC688 Datasheet download

Information published by TI regarding third-party products or services. By using our site, you acknowledge that you have read and understand our Cookie PolicyPrivacy Policyand our Terms of Service.

Production processing does not necessarily include. Sign up or log in Sign up using Google.

Supply voltage range, V CC. The outputs are measured one at a time with one input transition per measurement. The package thermal impedance is darasheet in accordance with JESD Resale of TI products or services with statements different from or beyond the parameters stated by TI for that. Customers are responsible for. I’m using a 74HC 8-bit magnitude comparator datasheet with Arduino. CL includes probe and test-fixture capacitance.

Exposure to absolute-maximum-rated conditions for extended periods may affect device reliability. Compare Two 8-Bit Words. The terminals are gold plated.

HC541 DATASHEET PDF

HC Reel of SN74HCPWT. –55 C to C. CDIP – J. Tube of 20 products and disclaimers thereto appears at the end of this data sheet. 1. 2. 3. 4. 74HC; 74HCT Octal buffer/line driver; 3-state. Rev. 4 — 3 March Product data sheet. Table 1. Ordering information. Type number. Package. HC Datasheet, HC PDF, HC Data sheet, HC manual, HC pdf, HC, datenblatt, Electronics HC, alldatasheet, free, datasheet.

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The outputs are measured one at a time with one input transition per measurement. Please be aware datwsheet an important notice concerning availability, standard warranty, and use in critical applications of. Refer to the TI application report. The package thermal impedance is hd541 in accordance with JESD All linear dimensions are in inches millimeters.

To minimize the risks associated with customer products. Load Circuit and Voltage Waveforms. TI is not responsible or liable for.

All products are sold subject to TI’s terms.

HC Datasheet catalog

Resale of TI products or services with statements different from or beyond the parameters stated by TI for that. A Max I CC. This drawing is subject to change without notice.

TI does not warrant or represent that any license, either express or implied, is granted under any TI patent right. Waveform 2 is for an output with internal conditions such that the output is high except dstasheet disabled by the output adtasheet. To Seven Other Channels. All linear dimensions are in millimeters. Reproduction of information in TI data books or data sheets is permissible only if reproduction is without.

Texas Instruments Incorporated and its subsidiaries TI reserve the right to make corrections, modifications. Exposure to absolute-maximum-rated conditions for extended periods may affect device reliability. Information published by TI regarding third-party products or services. Testing and other quality control techniques are used to the extent TI.

[ Free Shipping ] HC541 74HC541 SN74HC541NSR new original

Low Power Consumption, ? The 3-state outputs are controlled by a two-input. Waveform 1 is for an output with internal conditions such that the output is low except when disabled by the output control. Body dimensions do not include mold flash or datasheeg not to exceed 0, TI assumes no liability for applications assistance or customer product design. The 20 pin end lead shoulder width is a vendor option, either half or full width.

The input and output voltage ratings may be exceeded if the input and output current ratings are observed. Phase relationships between waveforms were chosen eatasheet. Low Input Current of 1?

Texas Instruments semiconductor products and disclaimers thereto appears at the end of this data sheet. TI warrants performance of its hardware products to the specifications applicable at the time of sale in. Use of such information may require a license from a third party under the patents datsaheet other intellectual property.

These are stress ratings only, and. The terminals are gold plated.

Post Office Box Dallas, Texas Customers are responsible for. Except where mandated darasheet government requirements, testing of all. Production processing does not necessarily include. This package can be hermetically sealed with a metal lid. Body dimensions do not include mold flash or protrusion not to exceed 0. Storage temperature range, T stg.

SGS Thomson Microelectronics

Following are URLs uc541 you can obtain information on other Texas Instruments products and application. CL includes probe and test-fixture capacitance. These octal buffers and line drivers feature the. If either output-enable OE1 or OE2. On all other products, production.

Products conform to specifications per the terms of Texas Instruments.

C549B DATASHEET PDF

Product data sheet. Supersedes data of Apr Oct DISCRETE SEMICONDUCTORS. BC; BC NPN general purpose. Maximum ratings (TA = 25/C). Grenzwerte (TA = 25/C). BC BC BC / Collector-Emitter-voltage. B open. VCE0. 65 V. 45 V. 30 V. CB Datasheet pdf, CB PDF Datasheet, Equivalent, Schematic, CB Datasheets, CB Wiki, Transistor, Cross Reference, PDF Download,Free.

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CB Datasheet, PDF – Alldatasheet

More parts based on the root C: When you buy electronic components from 1-Source you can be sure you are getting only the best parts at the best prices. Low noise transistors bcb,c npn silicon bcb,c. C c transistor transistor c spra c, transistor c europe c b spru transistor f text.

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BC549 Datasheet, Equivalent, Cross Reference Search

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Bc bc bc bc bc npn epitaxial silicon transistor. Consignment and warehousing available. Lm adjustable precision zener shunt regulator datasheet. It has environmentally friendly modules with easy assemblage, solderfree options, and rohs compliance.

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LFL 1.322 PDF

Unitech Combustion – offering Siemens Gas Burner Sequence Controller LFL , LFL And V AC in Ahmedabad, Gujarat. Read about company. Flamco Combustions Private Limited – offering Siemens Gas Burner Controller, LFL in New Delhi, Delhi. Read about company and get contact details and . UNITECH COMBUSTION – Manufacturer & Supplier of Siemens Controller LFL from Ahmedabad, Gujarat, India. Find India’s Best Quality Siemens.

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Gas Burner Control Box.

Year of Establishment The item may have some signs of cosmetic wear, but is fully operational and functions as intended. For universal use with multistage or modulating Delivery times may vary, especially during peak periods. At the time of procurement, we ensure that these products are manufactured as per defined industry standards through extensive ll test measures.

See all condition definitions – opens in a new window or tab The seller won’t accept returns for .1322 item. Gas Burner Control Box in Mumbai. For intermittent operation at least one controlled shutdown every 24 hours.

Siemens Gas Burner Controller, LFL , Flamco Combustions Private Limited | ID:

Learn More – opens in a new window or tab International postage and import charges paid to Pfl Bowes Inc. This item will post to United Statesbut the seller hasn’t specified postage options.

For use with dual fuel burners. I agree to the terms and privacy policy.

No additional import charges on delivery. Learn more – opens in a new window or tab. Select a valid country. With a superior quality product range, lfll serve varied industry segments such as Heat Generators, Boilers, Furnaces, and Chemicals.

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Siemens Gas Burner Controller, LFL 1.322

An item 1.32 has been previously used. This amount is subject to change until you make payment. Read more about the condition. Owing to our rich vendor base, we are able to import and supply superior quality Denmark, Italyand France made products.

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Learn More – opens in a new window or tab Any international postage and import charges are paid in part to Pitney Bowes Inc. Seller assumes all responsibility for this listing. Get best deals for coconut.

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JOHANNA LINDSEY WHEN LOVE AWAITS PDF

Editorial Reviews. Review. “Johanna Lindsey creates fairy tales that come true”–” Romantic Times”. About the Author. One of the world’s most successful authors. When Love Awaits by Johanna Lindsey – book cover, description, publication history. When Love Awaits By Johanna Lindsey – FictionDB. Cover art, synopsis, sequels, reviews, awards, publishing history, genres, and time period.

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Detailed Review Summary of When Love Awaits by Johanna Lindsey

He left immediately in the morning, and was away trying to seize one of his 7 castles. If you’re looking for a Medieval romance with a hero who is pretty much a puppy in the best of ways!

Rolfe has fought many battle, he has over seven keeps, close friends with the current King of England, his ward is his mistress who runs his household, and decides to marry Lady Leonie out of revenge. Brave the Wild Wind.

See all 71 reviews. With both of them having wrong impressions of each other; Leonie thinking Rolfe a cruel and selfish man who takes what he wants without worrying about the consequences, and Rolfe thinking that Leonie had set her servants onto him by destroying his land, their marriage was never going to be off to a good start. Bukan contoh hero yang baik nih Read the translated version and write the review in my own languange Buku yang bikin emosi again Emang ada aja yah tokoh cowo yang nyimpen mistressnya, sementara istrinya ada di tempat yang sama Looking for beautiful books?

Review quote “Johanna Asaits creates fairy tales that come true”–” Romantic Times” show more.

By using our website you agree to our use of cookies. He falls hard and fast for her and remains faithful to her since their marriage, and along the way he also courts her and longs for the affection of a woman in love from her.

Mar 18, ShortyS rated it it was ok Shelves: Duchess in Love Eloisa James. AmazonGlobal Ship Orders Internationally. And I absolutely love love love him! Lindsey lives in New England with her family. Leonie knows Rolfe only linsdey his bad reputation, but she will not defy the king. Leonie, is a amazing woman and was very aptly descripted in When Love Awaits. Refresh and ,indsey again. There was a problem filtering reviews right now. You can aeaits the unavailable item s now or we’ll automatically remove it at Checkout.

Get fast, free shipping with Amazon Prime. Spine creases, wear to binding and pages from reading.

International customers will be reimbursed a portion of unused shipping charges. Buy the selected items together This item: During their wedding day, they barely say two words to each other. Living alone with her people away from the suitors she might have had, Leonie begins to think she is happy in that state.

The hero and heroine had problems – for the entirety of the book no, really, the entire book is just that – which could be easily resolved if they would just talk to each other. I just didn’t get why they arrived at so many conclusions from so unreliable sources. Lady Leonie grew up awajts a mother, her father remarried, and her step mother ends up controlling everything in the household when she drugs her father enough to appear drunk.

When Love Awaits

Marry Me by Sundown. Great condition for a used book! The heroine, Leonie, is both gullible AND stupid. Once Lady Leonie cursed ‘the Black Wolf’ – handsome Rolfe d’Ambert, the mercenary Lord of Kempston – for his bloody deeds and cruelty to the local peasants.

When Love Awaits Book Summary and Study Guide

Cover To Cover Books, Inc. Then she is recused before it even starts.

Click here to see the rest of this review. Trivia About When Love Awaits. Because he misses her. M and N Media Condition: Now she must marry the magnificent blackguard to bring peace to the land. A really strong heroine who does not need to dress up as a man to assert her strength.

CRITERIOS DE APACHE PARA PANCREATITIS PDF

1) APACHE II > 11 en las primeras 24 horas 2) Lavado peritoneal con CRITERIOS DE SEVERIDAD DE BALTHAZAR-RANSON PARA TC. Online calculator for the Acute Physiology and Chronic Health Evaluation ( APACHE II) to predict hospital mortality based on 14 factors. The Pearson correlation (PC) for APACHE-II and Ranson p = of de severidad de la pancreatitis aguda según criterios de Ranson, APACHE-II y.

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Complete data on all 12 physiologic measurements was only available for patients. Concerning the hematocrit value, 57 and In table IIwe can observe the characteristics of the patients according to the severity markers. Retrieved from ” https: For a better determination of the disease’s severity, it must be performed 2 to 3 days after the beginning of the symptoms.

ds

History of severe organ failure or immunocompromise. It has not been validated for use in children or young people aged under Arterial blood gas Catheter Arterial line Central venous catheter Pulmonary artery catheter Blood cultures Screening cultures.

But drawing thresholds is a losing method when you have a continuous measure, like blood lactate. This score can be calculated on all patients newly admitted to the intensive care unit.

Heart Failure Class IV, cirrhosis, chronic lung disease, or dialysis-dependent. Risk prediction of hospital mortality for critically ill hospitalized adults”. Or create a new account it’s free. If the CT is performed before this period, the results may be lower Balthazar degrees.

On this study we found that in our hospital service we have a pancrfatitis frequency of the disease. Hemodynamics Hypotension Level of consciousness Acid—base imbalance Water-electrolyte imbalance. We found a similar distribution between the slight and severe disease: So we started looking at the role of using physiology of a patient in the intensive care unit and to then develop a comprehensive measure of severity that could at least begin to discriminate one patient from another better than the DRG.

In order to calculate a mortality risk, the patient’s indication for ICU admission must be accounted for.

APACHE II Score – MDCalc

The diagnosis of acute pancreatitis was established with 2 of the 3 following criteria: Rev Esp Enferm Dig ; The area under the ROC curve in the entire population was 0. Within them, the measurement of reactive C protein must be taken into account.

Independent validation of APACHE II severity of illness score for predicting mortality in patients with breast cancer admitted to the intensive care unit. Must be evident prior to hospitalization. In order to see the staging of pancreatic damage, these patients had performed an abdominal tomography 72 hours after the beginning of the symptoms.

This article includes a list of referencesbut its sources pancreaittis unclear because it has insufficient inline citations. Health science Medicine Medical specialities Respiratory therapy. The study did not have a pre-defined validation population, so validation occurred within the original training group.

APACHE II Calculator

The age average was An important consideration was the impossibility to correlate the tomographic finds with the serum concentration of reactive C proteins, which is considered until pancreatitiis present moment the best prognosis indicator of AP. As it is pointed in some studies, the APACHE-II scale at the moment of admission is not to be trusted to neither diagnose pancreatic necrosis nor severe pancreatitis Am J Gastroenterol ; There were included patients of any gender above the age of 18, crietrios diagnosis of acute pancreatitis of any etiology, pancretaitis had performed an abdominal tomography 72 hours after the beginning of the clinical condition in order to stage the pancreatic damage.

The patient population was mostly comprised of surgical patients however. It is not calculated sequentially and is not meant to show improvement or effect of interventions. Central tendency measurements and dispersion for the quantitative variables were used; the frequencies are expressed in proportion terms and written between parentheses.

Biopsy-proven cirrhosis with portal hypertension; episodes of past upper GI bleeding attributed to portal hypertension; or prior episodes of of hepatic failure, encephalopathy, or coma Cardiovascular: They can also be used to determine prognosis and help family members make informed decisions about the aggressiveness of care. Subcategory of ‘Diagnosis’ designed to be very sensitive Rule Out.

Apuntes de Medicina Intensiva

The point score is calculated from a patient’s age and 12 routine physiological measurements:. The score is not recalculated during the stay; it is by definition an admission score. Medical non-operative Emergency post-operative Elective post-operative. Early onset of organ failure is the best predictor of mortality in pancreatktis pancreatitis.

Calc Function Calcs that help predict probability of a disease Diagnosis.

While it panvreatitis not mandatory and will not help with patient management, it is a useful tool for risk stratification and to compare the care received by patients with similar risk characteristics in different units. The AP diagnosis was performed to the patients that had at least 2 of the 3 following criteria: The area under the ROC curve was higher than 0.

CHANSON TRISTE TCHAIKOVSKY CELLO PDF

Print and download Chanson Triste, Op. 40, No. 2 – Cello & Piano sheet music composed by Peter Ilyich Tchaikovsky arranged for Cello or Piano. Sheet Music – £ – This famous piece by Tchaikovsky is for Cello (or Violin) and Piano arranged by Johannes Palaschko. Seperate Cello and Violin parts are . Chanson Triste “Tchaikovsky” Cello: Annita Piano: Samuel Alemán.

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Editor Gustav Saenger OK, I agree No, give me more info. The score can be downloaded in the format of your preference: Try again shutterfreak worked hard on this score. Scanned at dpi, cleaned with 2-point algorithmadditional manual cleaning. Allegro giusto G major, bars Chanson triste. To print parts, please upgrade to a Pro account first. Follow to get notified when shutterfreak has uploaded new scores. Editor Anatoly Drozdov — Naxos Javascript not enabled.

Why am I seeing this? Tempo di Mazurka C major, bars Mazurka. Creative Commons Attribution 4. We use cookies on this site to enhance your user experience By clicking any link on this page you are giving your consent for us to set cookies.

Tchaikovsky – Chanson Triste sheet music for Cello

Pieces ; For piano ; Scores featuring the piano ; For 1 player ; For organ arr ; Scores featuring the organ ; For harmonium arr ; Scores featuring the harmonium tchaikovssky For Wagner tuba, piano arr ; Scores featuring the Wagner tuba ; For 2 players ; For violin, piano arr ; Scores featuring the violin ; For viola, piano arr ; Scores featuring the viola ; For cello, piano arr ; Scores featuring the cello ; For guitar arr ; Scores featuring the guitar.

Want to give something back? Go Pro Upload Log In. Tempo di Marcia tchaiiovsky C minor, bars Mazurka. Andante sostenuto A minor—C major Valse. Arranger August Reinhard Le Service de l’organiste, Op.

Tchaikovsky – Chanson triste (for piano and cello) sheet music download free in PDF or MIDI

Neue Musik-Zeitung Unidentified Publishern. Your download has started.

Allegro moderato A minor, bars Au village. Creative Commons Attribution 3. You can select several formats for downloading the score. Plates to Tchaikovsky Pages 3 Duration Choose where you want to share: Contents 1 Performances 1. Retrieved from ” http: Recordings Scores published by D.

Andantino A minor, bars Scherzo. This file is part of the Sibley Mirroring Project. This file is based on high-resolution images obtained from the source using a method explained on this page. Creative Commons Zero 1. Link to this page Embed on your site.

Give a small token of appreciation! That’s what I want to ask too. Work Title 12 Pieces Alt ernative.

Originally written for violin and piano, this melancholic piece has been transcribed for cello and piano from a score found at FluteTunes. This file is from the MIT archive project. PDF scanned by P. Uploaded on Oct 16, Duration 43 minutes Composer Time Period Comp.

Peter Tchaikovsky – Chanson triste, Op. 40 No. 2 (cello & piano)

Arranger Friedrich Hermann Arrangements and Transcriptions Chanson triste No. Javascript is required for this feature. Try again More of this, please?

I added articulations for the cello, and put the score in tenor clef for practical reasons. Carl Fischer There’s a download link to the right of the page.

Part of “Twelve pieces”, Op 40 no 2. Album for Viola and Piano, Book 2, No.

ANTITUSIGENOS OPIACEOS PDF

Protozoos Helicobacter Pylori Giardia lamblia. Blastocitis Hominis Trichomona Vaginalis Ameba Histolica Toxoplasmosis Helmintos. fin de reflejar la diferencia en la utilización de esas drogas como analgésicos y como antitusígenos. Algunos opiáceos tienen indicaciones de uso diversas. sustancias como analgésicos y como antitusígenos. En el caso de la los opiáceos codeína, dihidrocodeína, etilmorfina e hidrocodona se pueden usar como.

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Efectos adversos Guayfenesina Jarabe.

Antiparasitarios, Antimicóticos, Antihipertensivos, Antitus by Patricia Pérez on Prezi

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ANTITUSIGENOS PDF DOWNLOAD

Atrapan, Destruir y Transportar. Hipersensibilidad a la antitusigenos o anhitusigenos.

Profilaxis antitusigenos y postoperatoria de complicaciones broncopulmonares. Antitusigenos, Destruir y Transportar. Send this link to let others join your antitusigenod Cancel Reply 0 characters used from the allowed. Send link to edit together this prezi using Prezi Meeting learn more: Ataques agudos de asma. Comments 0 Please log in to add antitusigenos comment.

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CULTIVO DE PROTOPLASTOS PDF

El cultivo de protoplastos en cítricos y su potencial para el mejoramiento genético. Article (PDF Available) · July with 1, Reads. Export this citation. Results 80 – 90 Download Citation on ResearchGate | On Jan 1, , R.R. Rodríguez and others published Aislamiento y cultivo de protoplastos en maracuyá. Universidad de León – Grado en Biotecnología – Tercero Biotecnología – Biotecnología vegetal – Tema 9. Cultivo de protoplastos e hibridación somática.

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Microcalli were transferred onto MS medium to induce shoot regeneration under indirect light conditions. Acknowledgments The authors would like to thank the International Potato Center for financial support and to Martha Williams de Castro who revised the English version of this article.

This study pgotoplastos that somatic hybrid potato plants can be obtained by the fusion method presented. When colonies developed into microcalli, the alginate beads were depolimerized. Then the suspension was centrifugated at rpm during ten minutes.

Tema 9. Cultivo de protoplastos e hibridación somática

Chromosome observations indicated that all hybrids were aneuploids. Root tips were stained by lacto-propionic orcein. Avoid resits and achieve higher grades with the best study guides, textbook notes, and class notes written by your fellow students. After seven days, the culture medium was diluted 1: Transfer of resistance to potato leaf roll virus from Solanum brevidens into Solanum tuberosum by somatic fusion.

These barriers in classical breeding can be overcome using biotechnological methods such as somatic hybridization by protoplast fusion Carputo et al. A rapid calli proliferation was observed and most of them showed purple-colored cells Fig. A revised medium for rapid growth bioassays with tobacco tissue culture. Potato, Solanum tuberosum L. These plants were multiplied in glass culture tubes for further analysis. Plant Cell Rep 3: Earn hundreds of dollars each month by selling your written material to your fellow students.

The other parent, the wild species S. Somatic potato hybrids have been produced by fusion of diploid wild species protopladtos tetraploid S. Somaclonal variation in potato. Aggregations of more than two protoplasts were also observed. Characterization of somatic hybrids of potato by use of RAPD markers and isozyme analysis. These samples were obtained from rooted plants maintained in a greenhouse. This value is different from that obtained by Mattheij et al.

Plant Cell Tissue Organ Cult. Protoplasts were precipitated by centrifugation at rpm during five minutes. Results and discussion Protoplasts fusion and plant regeneration Protoplasts fusion from S.

Shoot regeneration started twelve weeks after calli were transferred to MS medium. Tissue culture response in various wild and cultivated Solanum germplasm accessions for exploitation in potato breeding.

Isolamento e cultivo de protoplastos de porta-enxertos de citros

In this paper we describe the fusion of leaf protoplasts of S. Plant Cell Rep Non-transgenic applications of plant tissue protolastos in potato.

This technique has created novel cellular genome configurations by combining sexually incongruent species. The morphology of fusion-derived regenerants was intermediate between the donor parents. Prior to fusion, protoplasts from S. This can be done via morphological, biochemical, cytological and molecular markers Pinto et al. To select hybrids, regeneration of in vitro plants was subjected to RAPD analysis.

Nineteen somatic hybrid plants were obtained and confirmed by RAPD analysis. Production of somatic hybrids between frost – tolerant Solanum commersonii and Solanum tuberosum: RAPD and chromosomal analysis of the hybrids are also presented. After protoplasts fusion and during plant regeneration it frequently happens that genetic changes such as variations in chromosome number occur.

Interspecific hybridization between the cultivated potato Solanum tuberosum subspecies tuberosum L. These results showed that all hybrids were aneuploids Fig.

AISLAMIENTO y CULTIVO DE PROTOPLASTOS EN MARACUYA by juank quintero on Prezi

rpotoplastos Using chemical or electrical procedures Jones, protoplasts from different donor plants can be fused together and somatic hybrids regenerated from the fusion products.

Many wild Solanum species are regarded as important sources for disease resistance and tolerance to many abiotic stresses Hawkes, but their use in potato breeding is limited due to poor crossability and sterility of interspecific hybrids.

A rapid calli proliferation was observed after colonies developed. The enzyme treatment was carried out overnight with gentle agitation. Segundo curso Primer curso.

Thanks to the study guides written by fellow students specifically for your courses, you will never miss a trick when it comes to your exams. In conclusion, somatic fusions between the cultivated potato Solanum tuberosum and the wild species S. For these reasons and considering the obtained results, it can be suggested that there is a high probability of fusion-derived regenerants which are aneuploids with an increase or loss of some chromosomes when one or both fusion partners have a high ploidy level.

Successful application of the fusion technique demands a protocol for plant regeneration from protoplasts. Isolation of plant DNA from fresh tissue. Interspecific somatic hybridization between Solanum tuberosum L.